v08b1 229e full length spike protein sino biological  (Sino Biological)

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Longitudinal antibody response to SARS-CoV-2, HCoV-OC43, and HCoV-229E proteins in the systemic compartment Serum from participants was analyzed at baseline and at days 3, 7, and 28 post-enrollment. (A) IgG, IgM, and IgA response for SARS-CoV-2 full-length protein and S1, S2, and RBD domains according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. (B) IgG, IgM, and IgA response for HCoV-OC43 full-length protein and S1 and S2 domains according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. (C) IgG, IgM, and IgA response for HCoV-229E full-length protein according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. ∗ p ≤ 0.05. Data on antibody were compared by multiple comparison analysis (Kruskal-Wallis test); if multiple comparison was statistically significant (∗ p ≤ 0.05), a pairwise comparison was performed and the Bonferroni correction for multiple tests was applied. Data on two-by-two comparison are shown in .

Article Snippet: OC43 full-length spike protein , Sino Biological , Cat#40607-V08B.

Techniques: Comparison

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Fold induction of SARS-CoV-2 and HCoV-OC43 S1 and S2 domains Boxplot diagrams of geometric mean fold rise antibody titers against SARS-CoV-2 and HCoV-OC43 S1 and S2 domains at different time points (days 3, 7, and 28 post-enrollment) according to groups (index, positive cases, negative cases). (A) Box-and-whisker diagrams of fold induction IgG, IgM, and IgA titers against SARS-CoV-2 S1 and S2 domains at different time points according to groups (index, positive cases, negative cases). Box indicates IQR (Q1–Q3) with line indicating median and error bars indicating minimum and maximum. (B) Box-and-whisker diagrams of fold induction IgG, IgM, and IgA titers against HCoV-OC43 S1 and S2 domains at different time points according to groups (index, positive cases, negative cases). Box indicates IQR (Q1–Q3) with line indicating median and error bars indicating minimum and maximum. Kruskal-Wallis test was performed to compare differences at each time point between the subdomains. Statistical significance was considered when p < 0.05 (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: OC43 full-length spike protein , Sino Biological , Cat#40607-V08B.

Techniques: Whisker Assay

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Binomial logistic regression of preexisting SARS-CoV-2, HCoV-OC43, and HCoV-229E antibody levels and the probability of infection upon SARS-CoV-2 exposure in the CIDS cohort (A) Violin plot of IgG, IgM, and IgA baseline antibody titers (log10 AUC) for SARS-CoV-2-S, HCoV-OC43-S, and HCoV-229e-S full-length proteins in index, positive cases, and negative cases groups. Median (continuous black line), and IQR (Q1–Q3) values (discontinued lines) are represented. Pairwise comparisons adjusted by the Bonferroni correction for multiple test has been performed, and significance of ∗ p < 0.05 is shown. (B–D) Scatterplot diagrams showing the inverse relationship between the predicted probability of infection (PP infection, %) and immunoglobulin levels for the indicated SARS-CoV-2 antigens: S (full-length), S1, RBD, and S2; (C) HCoV-OC43: S (full-length), S1, and S2; and (D) HCoV-22E S (full-length). Logistic regression analysis has been performed for each variable individually, and significance of ∗ p < 0.05 is shown.

Article Snippet: OC43 full-length spike protein , Sino Biological , Cat#40607-V08B.

Techniques: Infection

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Multi-assay modeling of systemic signatures of protection against SARS-CoV-2 infection (A) Multivariate logistic regression model analyzing the potential protection of baseline antibodies against S1 versus S2 domains of SARS-CoV-2 virus for IgG, IgM, and IgA isotypes. (B) Multivariate logistic regression model analyzing the potential protection of baseline antibodies against S1 versus RBD domains of SARS-CoV-2 virus for IgG, IgM, and IgA isotypes. (C) Multivariate logistic regression model analyzing the potential protection of baseline antibodies against S1 versus S2 domains of HCoV-OC43 virus for IgG, IgM, and IgA isotypes. (D) Multivariate logistic regression model analyzing the potential protection of baseline antibodies against the S2 domain of HCoV-OC43 virus for IgG, IgM, and IgA isotypes. (E) Multivariate logistic regression model analyzing the potential protection of anti-S1 IgG SARS-CoV-2 and anti-S2 IgM HCoV-OC43 from SARS-CoV-2 infection. OR and 95% CIs are shown for all the variables. p values for the significant variables are shown. All the models are adjusted by potential confounding factors (age and sex).

Article Snippet: OC43 full-length spike protein , Sino Biological , Cat#40607-V08B.

Techniques: Infection, Virus

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Multi-assay modeling of mucosal signatures of protection against SARS-CoV-2 infection Nasopharyngeal swab samples from participants were analyzed at baseline and at days 3, 7, and 28 post-enrollment. (A–H) IgG, IgM, IgA, and secretory IgA (sIgA) response for (A) SARS-CoV-2 S and (B) HCoV-OC43 S proteins according to groups (index, positive cases, and negative cases) on the upper respiratory tract. Dots represent the GMT, and bars indicate 95% CI. Fold change antibody titers against (C) SARS-CoV-2 and (D) HCoV-OC43 S protein represented as boxplot. Box indicates IQR (Q1–Q3), with horizontal line at median and whiskers representing minimum and maximum. Binomial logistic regression of pre-existing (E) SARS-CoV-2 and (F) HCoV-OC43 antibody levels and the probability of SARS-CoV-2 infection. Scatterplot diagrams show the inverse relationship between the PP infection % and anti-SARS-CoV-2 S IgG, IgM, IgA, and sIgA mucosal levels. Multivariate logistic regression model analyzing the potential protection of (G) anti-SARS-CoV-2 S and (H) anti-HCoV-OC43 S IgG, IgM, IgA, and sIgA mucosal antibody levels at enrollment. (I) Multivariate logistic regression model analyzing the potential protection of mucosal anti-S IgG SARS-CoV-2 and anti-S IgA HCoV-OC43 antibody levels at enrollment from SARS-CoV-2 infection. (J) Mixed multivariate logistic regression analysis of the potential protection of systemic (SARS-CoV-2 S1 IgG and HCoV-OC43 S2 IgM) and mucosal (SARS-CoV-2 S IgG and HCoV-OC43 S IgA) antibody levels at enrollment for SARS-CoV-2 infection. Data on antibody were compared by multiple comparison analysis (Kruskal-Wallis test); if multiple comparison was statistically significant (∗ p ≤ 0.05), a pairwise comparison was performed and the Bonferroni correction for multiple tests was applied. Data on two-by-two comparison are shown in and .

Article Snippet: OC43 full-length spike protein , Sino Biological , Cat#40607-V08B.

Techniques: Infection, Comparison

Journal: iScience

Article Title: Adaptive immune responses against common viruses are sustained and functional in end-of-life patients

doi: 10.1016/j.isci.2025.112082

Figure Lengend Snippet: Virus-specific mCD4+ T cell responses are maintained at the end of life (A) Graphs showing the net frequency (left) and stimulation index (SI; right) of virus-specific mCD4+ T cells (CD69 + CD40L+) in end-of-life patients (OC43, n = 34; CMV, n = 18) and elderly controls (OC43, n = 28; CMV, n = 20). Dashed line indicates the threshold of a positive response (SI > 1.5). Mann Whitney U-test. (B) UMAP contour of bulk and virus-specific mCD4+ T cells generated from four representative, positive donors for each condition and cohort (OC43 patients, n = 4; OC43 controls, n = 4; CMV patients, n = 4, CMV controls, n = 4). (C) UMAP contour (gray) overlayed with the distribution of virus-specific mCD4+ T cells in end-of-life patients (purple) and elderly controls (black). (D) UMAP plots overlayed with the median fluorescence intensity for each of the measured markers. (E) UMAP plot overlayed with clusters annotated with PhenoGraph based on the measured markers. (F) Heatmap showing the hierarchical clustering of median relative expression of the indicated markers in the clusters defined in (E). Data are represented as median relative expression. (G) Graph showing the median distribution (bar) of OC43-specific and CMV-specific mCD4+ T cells for end-of-life patients and elderly controls across the clusters shown in (E), with individuals shown as filled circles with (CMV) or without a dot (OC43). Mann Whitney U test.∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001. The median (black line) is shown when applicable.

Article Snippet: HCoV-OC43 full length spike protein antigen , Sino Biologicals , Cat#40607-V08B.

Techniques: Virus, MANN-WHITNEY, Generated, Fluorescence, Expressing

Journal: iScience

Article Title: Adaptive immune responses against common viruses are sustained and functional in end-of-life patients

doi: 10.1016/j.isci.2025.112082

Figure Lengend Snippet: Phenotypic changes to virus-specific mCD4+ T cells have limited effect on functional capacity (A) Pie chart generated by SPICE showing the phenotypic profile of virus-specific mCD4+ T cells in end-of-life patients (OC43, n = 12; CMV, n = 16) and elderly controls (OC43, n = 21; CMV, n = 18). The white piece represents cells lacking expression of all included markers. Only donors with a positive antigen-specific response were included. Permutation test. Data are represented as mean frequency. (B) Graphs showing the frequency of virus-specific mCD4+ T cells from end-of-life patients (OC43, n = 12; CMV, n = 16) and elderly controls (OC43, n = 21; CMV, n = 18) expressing the measured phenotypic markers. Only positive donors were included. Mann Whitney U test. (C) Pie chart generated by SPICE showing the functional profile of virus-specific mCD4+ T cells in end-of-life patients (OC43, n = 12; CMV, n = 16) and elderly controls (OC43, n = 21; CMV, n = 18). The white piece represents cells lacking expression of all included markers. Only positive donors were included. Permutation test. Data are represented as mean frequency. (D) Graphs showing the frequency of virus-specific mCD4+ T cells in end-of-life patients (OC43, n = 12; CMV, n = 16) and elderly controls (OC43, n = 21; CMV, n = 18) expressing the measured functional markers. Only positive donors were included. Mann Whitney U test. (E) Graph showing the frequency of CMV-specific mCD4+ T cells expressing markers whose expression was, or close to, significantly different between end-of-life patients who survived ≤30 days ( n = 9) or >30 days ( n = 7) after sampling. Only positive donors were included. Mann Whitney U test.∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001. The median (black line) is shown when applicable.

Article Snippet: HCoV-OC43 full length spike protein antigen , Sino Biologicals , Cat#40607-V08B.

Techniques: Virus, Functional Assay, Generated, Expressing, MANN-WHITNEY, Sampling

Journal: iScience

Article Title: Adaptive immune responses against common viruses are sustained and functional in end-of-life patients

doi: 10.1016/j.isci.2025.112082

Figure Lengend Snippet: Virus-specific CD8 + T cells show a higher degree of activation in the end-of-life patient cohort (A) Graphs showing the net frequency (left) and stimulation index (right) of virus-specific mCD8+ T cells (CD69+IFN-γ+) in end-of-life patients (OC43, n = 33; CMV, n = 18) and elderly controls (OC43, n = 28; CMV, n = 20). Dashed line indicates the threshold of a positive response (SI ≥ 1.5). Mann Whitney U test. (B) UMAP contour of bulk mCD8+ T cells and CMV-specific mCD8+ T cells generated from four representative donors for each condition and cohort (patients, n = 4; controls, n = 4). (C) UMAP contour (gray) overlayed with the distribution of CMV-specific mCD8+ T cells in end-of-life patients (purple) and elderly controls (black). (D) UMAP plots overlayed with the median fluorescence intensity for each of the measured markers. (E) UMAP overlayed with clusters annotated with PhenoGraph based on the measured markers. (F) Heatmap showing the hierarchical clustering of median relative expression of the indicated markers in the clusters defined in (E). Data are represented as median relative expression. (G) Graph showing the median distribution (bar) of CMV-specific mCD8+ T cells within the clusters shown in (E), with individuals shown as filled circles with a dot. Mann-Whitney U test.∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001. The median (black line) is shown when applicable.

Article Snippet: HCoV-OC43 full length spike protein antigen , Sino Biologicals , Cat#40607-V08B.

Techniques: Virus, Activation Assay, MANN-WHITNEY, Generated, Fluorescence, Expressing

Journal: iScience

Article Title: Adaptive immune responses against common viruses are sustained and functional in end-of-life patients

doi: 10.1016/j.isci.2025.112082

Figure Lengend Snippet:

Article Snippet: HCoV-OC43 full length spike protein antigen , Sino Biologicals , Cat#40607-V08B.

Techniques: Staining, Recombinant, Software

Journal: Frontiers in Immunology

Article Title: Effect of seasonal coronavirus immune imprinting on the immunogenicity of inactivated COVID-19 vaccination

doi: 10.3389/fimmu.2023.1195533

Figure Lengend Snippet: Seroprevalence of sCoVs and SARS-CoV-2 S IgG antibodies in different cohorts.

Article Snippet: Briefly, to detect corresponding antibodies, 96-well EIA/RIA plates (Corning, #3590) were coated with recombinant SARS-CoV-2 full-length S (Sino Biological, #40591-V08B1-1), SARS-CoV-2 S1(Sino Biological, #40591-V08H), S2 (Sino Biological, #40590-V08B), SARS-CoV-2 full-length N (Sino Biological, #40588-V08B), HCoV-HKU1 full-length S (Sino Biological, #40606-V08B), HCoV-NL63 full-length S (Sino Biological, #40604-V08B), HCoV-OC43 full-length S (Sino Biological, #40607-V08B), and HCoV-229E full-length S proteins (Sino Biological, #40605-V08B) at 100ng/ul in ELISA coating buffer (Solarbio, #C1055) respectively, and incubated at 4°C overnight.

Techniques:

Journal: Frontiers in Immunology

Article Title: Effect of seasonal coronavirus immune imprinting on the immunogenicity of inactivated COVID-19 vaccination

doi: 10.3389/fimmu.2023.1195533

Figure Lengend Snippet: Seroprevalence of sCoVs and SARS-CoV-2 S IgG antibodies in different cohorts.

Article Snippet: Briefly, to detect corresponding antibodies, 96-well EIA/RIA plates (Corning, #3590) were coated with recombinant SARS-CoV-2 full-length S (Sino Biological, #40591-V08B1-1), SARS-CoV-2 S1(Sino Biological, #40591-V08H), S2 (Sino Biological, #40590-V08B), SARS-CoV-2 full-length N (Sino Biological, #40588-V08B), HCoV-HKU1 full-length S (Sino Biological, #40606-V08B), HCoV-NL63 full-length S (Sino Biological, #40604-V08B), HCoV-OC43 full-length S (Sino Biological, #40607-V08B), and HCoV-229E full-length S proteins (Sino Biological, #40605-V08B) at 100ng/ul in ELISA coating buffer (Solarbio, #C1055) respectively, and incubated at 4°C overnight.

Techniques: